The cleavage was significantly promoted after theophylline adding
The maximum magnitude of the Similarly, appending the anti-theophylline aptamer to a small ribozyme ligase (L1) generated an aptazyme that was activated 1,600-fold by saturating theophylline concentrations
The active theophylline aptazyme cleaves itself, witch prevents the reporter gene transcription
Substantial We chose theophylline as the exogenous ligand of hammerhead aptazyme because it is a commonly used medicine for the treatment of chronic asthma with a good safety profile
To date, aptamers that bind theophylline,4−7 tetracycline,8,9 thiamine pyrophosphate,10,11 and guanine12,13 have been used Aptazyme library Lib-2: the guanine aptamer (blue) and the ribozyme were fused in tandem
gene expression and safety control is also involving the modulation of theophylline-dependent HHAz variants
A, Sequence and secondary structure of the theophylline-inducible aptazyme (aTheoAz) built in VARNA (Darty et al
In another study, the theophylline aptamer of an ON-switching aptazyme was replaced by a tetracycline aptamer resulting in a new switch with even increased